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Molecular Analyses
Molecular Analyses 1st Edition (Molecular Genomics and Proteomics):
DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the major audience will be medical professionals, students, and those involved in medically-related research endeavors. There are also papers in this volume dealing specifically with methods developed to analyze large sequence data sets. Many methods reviewed herein are more broadly applicable to other fields in biology, chemistry, bioinformatics, and bioengineering, and are intended for a broad readership.
Key Features
Summarizes nucleic acid extractions from a wide variety of tissues and cells
Describes processes of nucleic acid preservation
Reviews forensic sampling, detection of nucleic acids, and delivery of nucleic acids to multicellular organisms
Provides essential guidance for sequencing, sequence analysis, database searches, and phylogenetic analyses
Includes additional methods useful for analysis of nucleic acids and proteins
Related Titles
DeSalle, et al. Phylogenomics: A Primer (ISBN 978-0-3670-2849-7).
Jennings, W. B. Phylogenomic Data Acquisition: Principles and Practice (ISBN 978-0-3678-6980-9).
Wang, X. Next-Generation Sequencing Data Analysis (ISBN 978-1-4822-1788-9)
Sung, W.-K. Algorithms for Next-Generation Sequencing (ISBN 978-0-3676-5797-0)
Additional ISBNs:
∗ eText ISBN: 1000596419, 978-1000596410, 9781000596410
- See additional information on the Amazon.
More Details
Molecular Analyses 1st Edition:
Cover Page
Half-Title Page
Series Page
Title Page
Copyright Page
Contents
Preface
List of Contributors
Chapter 1 Nucleic Acid Extraction from Diverse Samples
Chapter 2 DNA Extraction from Mummified Tissues
Chapter 3 Commercial DNA Extraction Kits
Chapter 4 Automated Nucleic Acid Extraction
Chapter 5 Forensic DNA Samples: Collection and Handling
Chapter 6 DNA Preservation
Chapter 7 RNA Storage
Chapter 8 Disposable Electrochemical DNA Biosensors
Chapter 9 Handheld Nucleic Acid Analyzer
Chapter 10 DNA-Binding Fluorophores
Chapter 11 Fluorescence Resonance Energy Transfer (FRET)
Chapter 12 LightUp® Probes
Chapter 13 Invader® Assay
Chapter 14 Laser-Capture Microdissection
Chapter 15 Helper-Dependent Adenoviral Vectors
Chapter 16 Retroviral Vectors
Chapter 17 Liposomal Nonviral Delivery Vehicles
Chapter 18 Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing
Chapter 19 Automated DNA Sequencing
Chapter 20 DNA Sequencing Methods
Chapter 21 Differential Sequencing by Mass Spectrometry
Chapter 22 Brief Guide to Conducting Biological Database Searches
Chapter 23 Accessing Genomic Databases
Chapter 24 Phylogenetics, Comparative Genomics, and Phylogenomics
Chapter 25 DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences
Chapter 26 ESTAnnotator: A Tool for High-Throughput EST Annotation
Chapter 27 The Basics of Omics
Chapter 28 Ribotyping
Chapter 29 Forensic DNA Typing: Y Chromosome
Chapter 30 Forensic Identification: An Overview on Molecular Diagnostic Technology
Chapter 31 Denaturing Gradient Gel Electrophoresis (DGGE)
Chapter 32 Heteroduplex Analysis (HA)
Chapter 33 NEBcutter: A Program to Cleave DNA with Restriction Enzymes
Chapter 34 Differential Display (DD) Analysis
Chapter 35 Protein Truncation Test (PTT)
Index
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