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Microbiology: A Laboratory Manual 12th Edition
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Microbiology: An Introduction 13th Edition
Microbiology: An Introduction 13th Edition $8.00
Pearson

Microbiology: A Laboratory Manual 12th Edition

$8.00

Format

PDF

Author(s)

James Cappuccino, Chad Welsh

Publisher

Pearson

ISBN-10

0135188997

ISBN-13

978-0135188996

Pages

560

Language

English

Edition

12th edition | February 1, 2019

File Size

35 MB

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$90

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ISBN: 978-0135188996 SKU: MB1513 Category: Microbiology Tags: Biology, Microbiology, Microbiology: A Laboratory Manual, Microbiology: A Laboratory Manual 12th Edition
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Description

Microbiology: A Laboratory Manual 12th Edition

Microbiology: A Laboratory Manual Twelfth Edition:

This loose-leaf, three-hole punched textbook that gives students the flexibility to take only what they need to class and add their own notes-all at an affordable price.
For courses in Microbiology Lab and Nursing and Allied Health Microbiology Lab.
Foundations in microbiology lab work with clinical and critical-thinking emphasis
Microbiology: A Laboratory Manual, 12th Edition provides students with a solid underpinning of microbiology laboratory work while putting increased focus on clinical applications and critical-thinking skills, as required by today’s instructors. The text is clear, comprehensive, and versatile, easily adapted to virtually any microbiology lab course and easily paired with any undergraduate microbiology text.
The 12th Edition has been extensively updated to enhance the student experience and meet instructor requirements in a shifting learning environment. Updates and additions include clinical case studies, equipment and material checklists, new experiments, governing body guidelines, and more.


Additional ISBNs:

∗ eText ISBN: 0135174619, 978-0135174616, 9780135174616

Table of Contents

Contents

Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural

Experiment 1: Effectiveness of Hand Washing

Experiment 2: Culture Transfer Techniques

Experiment 3: Techniques for Isolation of Pure Cultures

Experiment 4: Cultural Characteristics of Microorganisms

Part 2 Microscopy

Experiment 5: Microscopic Examination of Stained Cell Preparations

Experiment 6:Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

Part 3 Bacterial Staining

Experiment 7: Preparation of Bacterial Smears

Experiment 8: Simple Staining

Experiment 9: Negative Staining

Experiment 10:Gram Stain

Experiment 11: Acid-Fast Stain

Experiment 12: Differential Staining for Visualization of Bacterial Cell Structures

Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations

Experiment 13:Nutritional Requirements: Media for the Routine Cultivation of Bacteria

Experiment 14: Use of Differential, Selective, and Enriched Media

Experiment 15: Physical Factors: Temperature

Experiment 16: Physical Factors: pH of the Extracellular Environment

Experiment 17: Physical Factors: Atmospheric Oxygen Requirements

Experiment 18: Techniques for the Cultivation of Anaerobic Microorganisms

Experiment 19: Serial Dilution-Agar Plate Procedure to Quantitate Viable Cells

Experiment 20: The Bacterial Growth Curve

Part 5 Biochemical Activities of Microorganisms

Experiment 21: Extracellular Enzymatic Activities of Microorganisms

Experiment 22: Carbohydrate Fermentation

Experiment 23: Triple Sugar-Iron Agar Test

Experiment 24: IMViC Test

Experiment 25: Hydrogen Sulfide Test

Experiment 26: Urease Test

Experiment 27: Litmus-Milk Reactions

Experiment 28: Nitrate Reduction Test

Experiment 29: Catalase Test

Experiment 30: Oxidase Test

Experiment 31: Utilization of Amino Acids

Experiment 32: Genus Identification of Unknown Bacterial Cultures

Part 6 The Protozoa

Experiment 33: Free-Living Protozoa

Experiment 34: Parasitic Protozoa

Part 7 The Fungi

Experiment 35: Cultivation and Morphology of Molds

Experiment 36: Isolation of a Soil Fungal Species

Experiment 37:Morphology, Cultural Characteristics and Reproduction

Part 8 The Viruses

Experiment 38: Cultivation and Enumeration of Bacteriophages

Experiment 39: Isolation of Coliphages from Raw Sewage

Experiment 40: Propagation of Isolated Bacteriophage Cultures

Part 9 Physical and Chemical Agents for the Control of Microbial Growth

Experiment 41: Physical Agents of Control: Moist Heat

Experiment 42: Chemical Agents of Control: Chemotherapeutic Agents

Experiment 43: Determination of Penicillin Activity in the Presence and Absence of Penicillinase

Experiment 44: Chemical Agents of Control: Disinfectants and Antiseptics

Part 10 Microbiology of Food

Experiment 45: Microbiological Analysis of Food Products: Bacterial Count

Experiment 46: Isolation of Salmonella from Raw Meat

Experiment 47: Microbial Fermentation

PART 11 Microbiology of Water

Experiment 48: Standard Qualitative Analysis of Water

Experiment 49: Quantitative Analysis of Water: Membrane Filter Method

PART 12 Microbiology of Soil

Experiment 50: Microbial Populations in Soil: Enumeration

Experiment 51: Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates

Experiment 52: Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

PART 13 Bacterial Genetics

Experiment 53: Enzyme Induction

Experiment 54: Bacterial Conjugation

Experiment 55: Isolation of a Streptomycin-Resistant Mutant

Experiment 56: The Ames Test: A Bacterial Test System for Chemical Carcinogenicity

Experiment 57: Utilization of Bacterial Plasmids

Experiment 58: Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA

PART 14 Medical Microbiology

Experiment 59: Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries

Experiment 60: Normal Microbial Flora of the Throat and Skin

Experiment 61: Identification of Human Staphylococcal Pathogens

Experiment 62: Identification of Human Streptococcal Pathogens

Experiment 63: Identification of Streptococcus pneumoniae

Experiment 64: Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems

Experiment 65: Isolation and Presumptive Identification of Campylobacter

Experiment 66: Microbiological Analysis of Urine Specimens

Experiment 67: Microbiological Analysis of Blood Specimens

Experiment 68: Species Identification of Unknown Bacterial Cultures

PART 15 Immunology

Experiment 69: Precipitin Reaction: The Ring Test

Experiment 70: Agglutination Reaction: The Febrile Antibody Test

Experiment 71: Enzyme-Linked Immunosorbent Assay

Experiment 72: Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures

About the Author

James Cappuccino, Chad Welsh

In Memoriam: James G. Cappuccino  (1930–2018)

James G. Cappuccino is a retired professor emeritus of microbiology from the Department of Biology of the State University of New York at Rockland Community College in Suffern, New York. He received his B.S degree from Seton Hall University in 1951, his M.S degree (1955) and his Ph.D. (1957) in microbiology from Rutgers University in New Brunswick, New Jersey. He was the author and co-author of numerous papers in the area of cancer research, and was a member of the faculty of the Sloan Kettering Division of the Graduate school of Medical Sciences at Cornell University where he taught microbiology from 1957-1970. From there, he taught microbiology, parasitology and clinical chemistry at SUNY Rockland until 2008. He was awarded the status of emeritus professor in 2012. In 1991 he was the recipient of the Chancellor’s award from the State University of New York for Excellence in Teaching. He is an emeritus member of the American Society for Cancer Research (ASCR) and an emeritus member of American society for Microbiology (ASM). When not writing he enjoys spending time with his wife Elaine and their family at their summer home at the New Jersey shore. He also enjoys theater, literature, and the quiet hour in his wood working shop.

Chad T. Welsh holds a Ph.D. in Microbiology and Immunology from the University of Louisville, School of Medicine, also an M.S. and B.S. in Biology from Middle Tennessee State University.  Currently he is the Chair of the Division of Biological and Earth Sciences at Lindenwood University in St. Charles, MO where he has the privilege of teaching Microbiology, both for non-majors and majors, Cellular Immunology, Parasitology, and many other courses since 2010.  His research interests fall within bacteriology, eukaryotic cell biology, and immunology, focusing primarily on intracellular eukaryotic signals in response to pulmonary bacterial pathogens.  His mentored research projects with his students have spanned the interest areas of soil microbial ecology, immune stress responses in collegiate athletes, oral bacterial flora communities, and many others.

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