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Lab Manual and Workbook in Microbiology: Applications to Patient Care 12th Edition
Lab Manual and Workbook in Microbiology: Applications to Patient Care Twelfth Edition:
This lab manual emphasizes the basic principles of diagnostic microbiology for students preparing to enter the allied health field. Students are led through a series of exercises that allow them to learn basic microbiology techniques and practice safety in the laboratory and hospital environment. It is primarily oriented toward meeting the interests and needs of those who will be directly involved in patient care and wish to learn how microbiological principles should be applied in the practice of their professions.
The authors articulate the purposes and function of the clinical microbiology laboratory in the diagnosis of infectious diseases. Exercises illustrate the nature of laboratory procedures used for isolation and identification of infectious agents as well as the principles of asepsis, disinfection and sterilization. The role of the health professional is highlighted in regard to appropriate collection of clinical specimens and application of aseptic and disinfectant techniques as they relate to patient care.
Additional ISBNs:
∗ eText ISBN: 1260163989, 978-1260163988, 9781260163988
- See additional information on the Amazon.
Table of Contents
Cover
Title Page
Copyright
Contents
Preface
Orientation to the Microbiology Laboratory
Part One Basic Techniques of Microbiology
Exercise 1. The Microscope
Exercise 2. Handling and Examining Cultures
Exercise 3. Simple Stains and Wet-Mount Preparations
Exercise 4. Gram Stain
Exercise 5. Acid-Fast Stain
Exercise 6. Culture Media
Exercise 7. Streaking Technique to Obtain Pure Cultures
Exercise 8. Subculture Techniques
Part Two Destruction of Microorganisms
Exercise 9. Moist and Dry Heat
Exercise 10. The Autoclave
Exercise 11. Disinfectants
Exercise 12. Antimicrobial Agent Susceptibility Testing and Resistance
Part Three Diagnostic Microbiology in Action
Exercise 13. Primary Media for Isolation of Microorganisms
Exercise 14. Some Metabolic Activities of Bacteria
Exercise 15. Activities of Bacterial Enzymes
Exercise 16. Antigen Immunoassays for Detection and Identification of Microorganisms
Exercise 17. Principles of Nucleic Acid Assays and Multiplex Syndrome Panel Testing for the Diagnosis of Infectious Disease
Exercise 18. Principles and Applications of MALDI-TOF Mass Spectrometry for the Rapid Identification of Bacteria and Fungi
Exercise 19. Staphylococci
Exercise 20. Streptococci, Pneumococci, and Enterococci
Exercise 21. Haemophilus, Corynebacteria, and Bordetella
Exercise 22. Clinical Specimens from the Respiratory Tract
Exercise 23. The Enterobacteriaceae (Enteric Bacilli) and Other Clinically Important Gram-Negative Bacilli
Exercise 24. Clinical Specimens from the Intestinal Tract
Exercise 25. Urine Culture Techniques
Exercise 26. Neisseria and Spirochetes
Exercise 27. Clinical Specimens from Blood
Part Four Microbial Pathogens Requiring Special Laboratory Techniques; Serodiagnosis of Infectious Disease; Principles and Practices of Infection Prevention
Exercise 28. Anaerobic Bacteria
Exercise 29. Mycobacteria
Exercise 30. Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions
Exercise 31. Fungi: Yeasts and Molds
Exercise 32. Protozoa and Animal Parasites
Exercise 33. Serodiagnosis of Infectious Disease
Exercise 34. Principles and Practices of Infection Prevention
Selected Literature and Sources
Index
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